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Microarrays were incubated at 40C for 48 hours in a rotating carousel. Hybridizations to custom Agilent microarrays were completed as previously described Hughes et al. Nat Biotech , 19 4 Microarrays were washed to remove non-specific hybridized sample.

Afterwards, microarrays were dried in an ozone-free nitrogen chamber. Microarrays were scanned using the Agilent LP2 laser scanner. The scanner output is a Tiff file, which contains the quantitative hybridization data from each individual microarray. The Tiff files were then processed using Rosetta custom feature extraction software. Website More Info. Colbert Infrared Testing Labs. Certified Calibration Service Testing Labs.

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